In the paper electrophoresis the sample is applied on a strip of filter paper wetted with desired buffer solution. The ends of the strip are dipped into the buffer reservoirs in which the electrodes are placed. the electric current is applied allowing the molecules to migrate for sufficient time. The paper is removed, dried and stained with a dye that specifically colours the substances to be detected. The coloured spots identified by comparing with a set of standards run simultaneously. Whatman No. 1 filter paper is used in the separation of protiens.
The serum proteins are separated into five distinct bands.
The serum proteins are separated into five distinct bands.
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