This technique involves combination of the principles of electrophoresis and immunological reactions (antibodies). Immunoelectrophoresis is useful for the analysis of complex mixtures of antigens and antibodies.
The complex proteins of biological samples are subjected to electrophoresis. the antibody is then applied in a trough parallel to the electrophoretic separation. the antibodies diffuse and when they come in contact with antigens, precipitation occurs, resulting in the formation of precipitin bands which can be identified.
Source : Biochemistry by Dr. U. Satyanarayana
The complex proteins of biological samples are subjected to electrophoresis. the antibody is then applied in a trough parallel to the electrophoretic separation. the antibodies diffuse and when they come in contact with antigens, precipitation occurs, resulting in the formation of precipitin bands which can be identified.
Source : Biochemistry by Dr. U. Satyanarayana
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