Thin layer Chromatography (TLC)

The principle of the Thin Layer Chromatography is the same as described for paper chromatography (partition). Instead of paper the Thin Layer Chromatography of very finely powdered silica gel, alumina, polyacrylamide gel, starch gel or kieselguhr, bound to a glass or plastic plate. Mixture is spread as a thin layer on glass or plastic plates. The chromatographic separation is comparatively rapid in TLC. In case of adsorption thin layer chromatography, adsorbents such as activated silica gel, alumina, kieselguhr are used.

Source : Biochemistry by Dr. U. Satyanarayana